Genome-edited powdery mildew resistance in wheat without growth penalties
基因组编辑的小麦白粉病抗性没有生长不利后果
成長ペナルティのないコムギのゲノム編集うどんこ病抵抗性
성장 페널티가 없는 밀의 게놈 편집 흰가루병 저항성
Resistencia al mildiú polvoroso editada por genoma en trigo sin penalizaciones de crecimiento
Résistance à l'oïdium du blé modifiée par modification du génome sans pénalités de croissance
Геномно отредактированная устойчивость пшеницы к мучнистой росе без штрафов за рост
Shengnan Li 李盛楠 ¹, Dexing Lin 林德行 ² ³ ⁴, Yunwei Zhang 张韫玮 ² ³, Min Deng 邓民 ² ⁴, Yongxing Chen 陈永兴 ², Bin Lv ¹ ⁵, Boshu Li ² ³ ⁴, Yuan Lei ² ³ ⁴, Yanpeng Wang 王延鹏 ² ³, Long Zhao 赵龙 ² ⁴, Yueting Liang ¹ ⁵, Jinxing Liu 刘金星 ² ³, Kunling Chen 陈坤玲 ² ³, Zhiyong Liu 刘志勇 ² ⁴, Jun Xiao 肖军 ² ⁴ ⁶, Jin-Long Qiu 邱金龙 ¹ ⁵, Caixia Gao 高彩霞 ² ³ ⁴
¹ State Key Laboratory of Plant Genomics, Institute of Microbiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China
中国 北京 中国科学院种子创新研究院 微生物研究所 植物基因组学国家重点实验室
² State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China
中国 北京 中国科学院种子创新研究院 遗传与发育生物学研究所 植物细胞与染色体工程国家重点实验室
³ Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
中国 北京 中国科学院遗传与发育生物学研究所 基因组编辑研究中心
⁴ College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China
中国 北京 中国科学院大学现代农业科学学院
⁵ CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China
中国 北京 中国科学院生物互作卓越创新中心
⁶ CAS-JIC Centre of Excellence for Plant and Microbial Science, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China
中国科学院遗传与发育生物学研究所 中英植物和微生物科学联合研究中心
Disruption of susceptibility (S) genes in crops is an attractive breeding strategy for conferring disease resistance. However, S genes are implicated in many essential biological functions and deletion of these genes typically results in undesired pleiotropic effects. Loss-of-function mutations in one such S gene, Mildew resistance locus O (MLO), confers durable and broad-spectrum resistance to powdery mildew in various plant species. However, mlo-associated resistance is also accompanied by growth penalties and yield losses, thereby limiting its widespread use in agriculture.
Here we describe Tamlo-R32, a mutant with a 304-kilobase pair targeted deletion in the MLO-B1 locus of wheat that retains crop growth and yields while conferring robust powdery mildew resistance. We show that this deletion results in an altered local chromatin landscape, leading to the ectopic activation of Tonoplast monosaccharide transporter 3 (TaTMT3B), and that this activation alleviates growth and yield penalties associated with MLO disruption.
Notably, the function of TMT3 is conserved in other plant species such as Arabidopsis thaliana. Moreover, precision genome editing facilitates the rapid introduction of this mlo resistance allele (Tamlo-R32) into elite wheat varieties. This work demonstrates the ability to stack genetic changes to rescue growth defects caused by recessive alleles, which is critical for developing high-yielding crop varieties with robust and durable disease resistance.
